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Figure 1 | Respiratory Research

Figure 1

From: A key role for STIM1 in store operated calcium channel activation in airway smooth muscle

Figure 1

Expression and siRNA mediated knockdown of STIM. (a) Expression of STIM1 and STIM2 in human airway myocytes and cultured human bronchial epithelial cells (hbec) using RT-PCR. PCR products were sequenced to confirm expression. (b) Tiled arrays of immunofluorescent staining of STIM-1 in HASM cells treated with either 20 nM scrambled siRNA (left) or 20 nM siRNA targeted at STIM1 (right). The intensity of STIM1 staining was decreased in cells treated with STIM1 siRNA. (c) siRNA targeted knockdown of STIM1 mRNA assessed by quantitative PCR. Cells transfected with STIM-1 siRNA dose dependently reduced STIM1 mRNA levels (76.9 ± 7.5% at 20 nM) without affecting STIM2 mRNA. (d) siRNA targeted knockdown of STIM2 mRNA. Cells transfected with STIM2 siRNA reduced STIM2 mRNA levels (70 ± 8.3% at 20 nM) without affecting STIM1. In addition, transfection of 20 nM scrambled, non-silencing siRNA had no effect on STIM expression.

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