Pharmacological inhibition and immunolocalisation of CFTR in rat tracheal smooth muscle cells. A Iodide efflux response as a function of time evoked by cAMP agonists (10 μM forskolin + 30 μM genistein) and 10 μM CFTRinh-172. B Effect of 100 μM glibenclamide, 500 μM DPC, 500 μM DIDS, 100 nM calixarene and 10 μM CFTRinh-172 on the efflux stimulated by cAMP agonists in rat tracheal smooth muscle cells as indicated. Basal was vehicle alone. Data are presented as mean ± S.E.M. All experimental conditions have been repeated: n = 4. C Immunofluorescence study of CFTR in TSMC and D in the absence of primary antibody. α-smooth muscle actin is stained in red, CFTR in green and nucleus (TOPRO-3 staining) in blue. Scale bars are 10 μm.