Figure 5
From: Caveolin-1 and -2 in airway epithelium: expression and in situ association as detected by FRET-CLSM
Cav-immunoreactive epithelial cell types. (A-C) Rat. (A) Cav-1α and eNOS double-labeling. Cav-1α-immunoreactivity (red, arrow) was found in ciliated cells (asterisks), labeled by anti-eNOS antibody (green, arrowhead). (B) Brush cells, labeled with anti-villin antibody (red, arrows) were not immunoreactive for cav-2 (green, arrowhead). (C) Cav-1α-immunoreactivity (red, arrow) in columnar epithelial cells and basal cells. Secretory cells were labeled with anti-SP-D antibody (green, arrowheads). In few secretory cells (asterisk), we could not determine definitely if they express cav-1α, since the cav-1α-immunoreactivity was localized at the cell membrane and SP-D-immunoreactivity was localized in the cytoplasm. (D-F) Human bronchus. Ciliated (arrowheads) and basal cells (arrows) were immunoreactive for cav-1α. Cell types are identified by their typical morphology as seen in the CLSM transmitted light mode. Punctate labeling for cav-1α was localized at the basolateral membrane (doubled arrowhead) and in the apical area of the ciliated cell beneath the luminal plasma membrane (arrowheads). Epithelium = e