Skip to main content

Table 1 Primer pairs for RT-PCR. Base sequences and product size for determining Cx26, Cx43, Cx46 and β-actin mRNA expression in RTEC

From: Laminin-332 alters connexin profile, dye coupling and intercellular Ca2+ waves in ciliated tracheal epithelial cells

Gene Upstream Sequence Downstream Sequence BP
Cx26 5'-CTGTCCTCTTCATCTTCCGC-3' 5'-TACGGACCTTCTGGGTTTTG-3' 306
Cx43 5'-CATTGGGGGGAAGGCGTGAGG-3' 5'-AGCGCACGTGAGAGATGGGGAAG-3' 400
Cx46 5'-GGAAAGGCCACAGGGTTTCCTGG-3' 5'-GGGTCCAGGAGGACCAACGG-3' 332
β-actin 5'-CGTGGGCCGCCCTAGGCACCA-3' 5'-TTGGCCTTAGGGTTCAGGGGGG-3' 242