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Figure 8 | Respiratory Research

Figure 8

From: Peroxisome Proliferator-Activated Receptor α (PPARα) down-regulation in cystic fibrosis lymphocytes

Figure 8

Differential PPARα binding to PPRE in peripheral lymphocytes. PPARα DNA binding was analyzed via gel shift assay in peripheral lymphocytes of CF patients (CF, n = 11; M/F: 6/5) and healthy subjects (C, n = 11; M/F: 6/5). The DNA binding element was biotin-labeled. (A) A representative gel shift. Lane (-) represents the biotin-labeled DNA binding element, without the addition of nuclear extract. Lane 1: Lymphocytic control sample. Lane 2: Specific cold oligonucleotide binding competition assay: a 60-fold excess of cold synthetic PPRE was added. Lane 3: Unspecific cold oligonucleotide binding competition assay. A 60-fold excess of unspecific synthetic oligonucleotide was used. (B) Densitometry data derived from the gel shift assays are expressed as means and standard error. These data show that PPARα DNA binding activity of CF patients is reduced by 36% compared to healthy persons. ** Significantly different (p < 0.01). On top: representative bands from a control person and a patient.

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