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Table 3 Effects of cytokines, autacoids and leukotrienes on 5-LO pathway enzymes and LT receptors in HBFs.

From: Human bronchial fibroblasts express the 5-lipoxygenase pathway

 

Control*

EGF

Cytomix

LTB4

LTD4

BK

HIS

5-LO

9.1 ± 2.0

8.8 ± 2.4

9.4 ± 3.6

10.5 ± 3.2

9.5 ± 2.4

10.8 ± 2.9

6.4 ± 1.9

FLAP

11.4 ± 2.8

11.3 ± 4.2

12.2 ± 3.7

10.5 ± 2.2

11.8 ± 3.2

11.2 ± 2.7

11.3 ± 3.6

LTA 4 H

8.2 ± 2.0

9.3 ± 2.3

10.1 ± 2.4

9.2 ± 2.2

9.1 ± 1.9

5.8 ± 1.1

9.8 ± 2.5

LTC 4 S

1.5 ± 0.7

1.2 ± 0.3

0.8 ± 0.5

0.8 ± 0.3

1.0 ± 0.5

1.0 ± 0.3

1.1 ± 0.4

BLT1

8.8 ± 2.3

14.2 ± 5.0

13.5 ± 4.9

12.8 ± 4.6

10.9 ± 4.5

11.5 ± 4.2

11.2 ± 4.7

CysLT 1

6.7 ± 1.4

7.5 ± 2.1

14.3 ± 4.5

12.0 ± 5.7

7.8 ± 1.5

8.5 ± 1.4

9.9 ± 1.7

  1. * Fibroblasts were cultured for 24 hours with either EGF (10 ng/ml), a combination of IL-1β, TNF-α and IFN-γ (Cytomix, each at 10 ng/ml), LTB4 (10 nM), LTD4 (10 nM), bradykinin (BK, 10 μM), histamine (HIS, 10 μM) or control medium before staining with specific antibodies and analysis by flow cytometry.
  2. p = 0.047 versus Control
  3. p = 0.036 versus Control