Table 1 Primer sequences for RT-PCR analysis of 5-LO pathway enzymes and receptors in human bronchial fibroblasts.

5-LO

upper 5'-TAC ATC GAG TTC CCC TGC TAC-3'

lower 5'-GTT CTT TAC GTC GGT GTT GCT-3'

FLAP

upper 5'-GAT GCG TAC CCC ACT TTC CTC-3'

lower 5'-GAA TAT GCC AGC AAC GGA CAT-3'

LTA ₄ H

upper 5'-GCC CGA GAT AGT GGA TAC CTG-3'

lower 5'-TTT TTG CTC AAA GCG ATA GGA-3'

LTC ₄ S

upper 5'-CTG TGC GGC CTG GTC TAC CGT 3'

lower 5'-GGG AGG AAG TGG GCG AGC AG-3'

CysLT ₁

upper 5'-CCG CTG CCT TTT TAG TCA GTT TC-3'

lower 5'-GGG TAC ATA AGT CAC GCT GGA CA-3'

GAPDH

upper 5'-GCT TGT CAT CAA TGG AAA TCC-3'

lower 5'-AGG GGA TGC TGT TCT GGA GAG-3'