HIMF induces Flk-1, but not VEGF, expression in mouse endothelial cell line. Endothelial SVEC 4–10 cells were treated with HIMF for various concentrations and periods as indicated. Western blot for VEGF and real-time RT-PCR for Flk-1 expression were performed. (2A) HIMF administration had no impact on VEGF expression in SVEC 4–10 cells. (2B) HIMF induced Flk-1 transcript increase in SVEC 4–10 cells in a dose-dependent manner. Time-course study indicated that HIMF (40 nmol/L)-induced Flk-1 expression can be detected at 6 h, and persisted for 24 h. Triplicate experiments were performed with essentially identical results (n = 3).