SP-A inhibited Af-induced CD4+ T cell proliferation and release of IL-4 and IL-5. (A): SP-A (10 μg/ml) increased the number of cells in the G1 (black bars) and decreased them in the G3-G4 populations of CD3/CD4+ lymphocytes (% change from the medium-treated samples). Average of 3 independent experiments. (B): Af-induced production of IL-4 and IL-5 by sensitized lymphocytes (S/Medium) was reduced in the presence of 10μg/ml of SP-A (S/SP-A) to levels similar to naïve lymphocytes (N/Medium). Lymphocytes were stimulated by Af (1 μg/ml). IL-4 (grey bars), IL-5 (white bars) and IFN-γ (black bars). * p < 0.05 S/SP-A vs. S/Medium. Mean ± SEM of n = 4 experiments performed in triplicates..