Longitudinal analysis of MCP-1 levels and CCR2+CD4+T cells. Longitudinal analysis of (A) MCP-1 levels and (B) CCR2+CD4+ T cells in three consecutive bronchoalveolar lavage fluids (BALF) of four children with interstitial lung diseases, including two children with pulmonary alveolar proteinosis (P) and one child with cholesterol pneumonitis (CP). The child with cholesterol pneumonitis and one child with pulmonary alveolar proteinosis died by respiratory failure (†), while one child with pulmonary alveolar proteinosis stayed clinically stable. † symbolize the childen who died. MCP-1 levels were quantified in BALF by a multiplex, particle-based assay. Percentages of CCR2+CD4+ T cells were analyzed in BALF by flow cytometry.