Global expression profiling of theophylline response genes in macrophages: evidence of airway anti-inflammatory regulation

Table 1 Oligonucleotides for real-time quantitative RT-PCR

mRNA targets	Oligonucleotides (5'→3') ^a	Product size (bp)
IL-5	F180: ATAGCCAATGAGACTCTGAGGATTC	89
	R268: AGTGTGCCTATTCCCTGAAAGAT
IL-13	F155: TGAGGAGCTGGTCAACATCA	76
	R230: CAGGTTGATGCTCCATACCAT
IL-18	F211: GCTGAACCAGTAGAAGACAATTGC	94
	R304: CCAGGTTTCATCATCTTCAGCTA
IL-13Rα1	F495: GGAATACCAGTCCCGACACTAACT	93
	R587: GGCCTTCTCTAAAGATGTTTTCACA
IL-13Rα2	F44: GGCTATTTGAAGTCGCCATAACC	78
	R121: AGATTTAAAACCTTGATATTGCCTCTCT
TNF-α	F414: CTCGAACCCCGAGTGACAA	64
	R477: AGCTGCCCCTCAGCTTGA
VEGF-a	F1200: AACACACACTCGCGTTGCAA	69
	R1268: CGGCTTGTCACATCTGCAAGT
VEGF-c	F1193: AGATGCCTGGCTCAGGAAGA	74
	R1266: ATGTCATGGAATCCATCTGTTGAGT
GM-CSF	F119: GCCCTGGGAGCATGTGAA	78
	R196: TTCATCTCAGCAGCAGTGTCTCTA
TBP	F852: CACGAACCACGGCACTGATT	89
	R940: TTTTCTTGCTGCCAGTCTGGAC

^a F and R indicate forward and reverse primers, respectively. Numbers indicate the mRNA sequence position.

ISSN: 1465-993X