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Figure 2 | Respiratory Research

Figure 2

From: Alveolar macrophage-epithelial cell interaction following exposure to atmospheric particles induces the release of mediators involved in monocyte mobilization and recruitment

Figure 2

Densitometric analysis of bands on RPAs. (A): the density of the bands representing the mediator mRNAs in AM/HBEC co-cultures on autoradiographs such as that shown in Figure 1A was compared to that of the GAPDH mRNA band in the same lane and the resulting ratio (PM10; black bars) is shown as the percentage change from control values (white bars). The mean densitometric value confirmed that the mRNA levels of MIP-1β, GM-CSF, MCP-1, IL-6 and ICAM-1 at 2 h and those of MIP-1β, GM-CSF, M-CSF and ICAM-1 after 24 h exposure were significantly higher than control values. Values are means ± SE of six experiments representing the AM/HBEC co-culture group. (B): the mean densitometric value confirmed that the mRNA levels of GM-CSF, LIF and ICAM-1 at 24 h exposure were significantly higher than control values. Values are means ± SE of four experiments representing the HBEC mono-culture group. *p < 0.05 compared with control.

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