Figure 1From: Alveolar macrophage-epithelial cell interaction following exposure to atmospheric particles induces the release of mediators involved in monocyte mobilization and recruitmentRNase protection assay of mRNA expression by AM and HBEC. Representative autoradiographs of RNase protection assays (RPAs) showing mediator expression by AM/HBEC co-cultures, AM mono-cultures and HBEC mono-cultures after 2 and 24 h incubation in medium alone (control) or a 100 μg/ml of PM10 suspension (PM10). After 24 h exposure AM showed increased expression of LIF and ICAM-1 mRNA. Expression of GM-CSF, LIF and ICAM-1 mRNA by HBEC was increased by 24 h PM10 stimulation compared to their respective controls. MIP-1β, GM-CSF, M-CSF, MCP-1, IL-6, LIF and ICAM-1 mRNA expression by AM/HBEC co-cultures was increased 2 and/or 24 h after incubation with PM10 compared to control. L32 and GAPDH were used as controls for lane loading.Back to article page