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Figure 4 | Respiratory Research

Figure 4

From: Detection of reactive oxygen species in isolated, perfused lungs by electron spin resonance spectroscopy

Figure 4

Superoxide release from isolated perfused rabbit lungs afteraddition of phorbol-12-myristate-13-acetate (PMA) during normoxic ventilation. (A) ESR signal intensity during normoxic ventilation of rabbit lungs in the presence (+SOD) and the absence of SOD (-SOD). After 3 h, PMA was injected into the pulmonary artery, resulting in a concentration of 1 μM in the recirculating buffer fluid. The increase in ESR signal intensity was linear before and after addition of PMA. The insert shows the PMA effect with higher time resolution. (B) Changes in the increase rate of the ESR signal intensity (%) by comparison of values prior to and after addition of PMA to isolated rabbit lungs. In the +SOD group, SOD was present throughout the experiments. In two separate sets of experiments, a fiber oxygenator was used instead of the lung for oxygenation of the recirculating buffer fluid ("fiber oxygenator"). The fibre oxygenator experiments were performed either in the absence ("fiber oxygenator") or in the presence of 1 μM FeCl2 ("fiber oxygenator + FeCl2") in the buffer fluid. * significant difference between the +SOD and the -SOD group

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