Figure 3
Hypoxia-dependent superoxide release and vasoconstrictor responses in isolated perfused and ventilated rabbit lungs. During a total 3 h period of perfusion, the ventilator gas supply was switched to different oxygen concentrations every 30 min, using 1 %, 2.5 %, 5 %, 10 %, 16 % or 21 % O2 in a randomized mode. (A) Superoxide release. The rate of increase in ESR signal intensity turned out to be linear during the last 20 min of each ventilation period. Therefore, changes in the ESR signal intensity/min during the last 20 min of each ventilation period are given. In the +SOD group, 150 mU/ml superoxide dismutase (SOD) was present throughout the experiments. * significant differences between the +SOD and the -SOD group for the respective oxygen concentration. ** significant differences as compared to 21 % O2. (B) Vasoconstrictor response. The maximum increases in PAP (ΔPAP) are given for the different oxygen concentrations and are referenced to baseline (=normoxic) PAP values, which were 8.8 ± 0.6 mmHg