Expression profiles of hydrophobic surfactant proteins in children with diffuse chronic lung disease

Respiratory Research

Table 2 Pro-SP-B and pro-SP-C in the comparison groups, i.e. children without lung disease and in children with chronic bronchitis.

	pro-SP-B							pro-SP-C
Detecting antibody	CTERMB				CFLANK	NFLANK	NFPROX
M_r of band	40–42	34–36	25–26	19–21	9	25–26
Children without lung disease (n = 15)	7% [8]	0%	80% [1,3,4,6–8,10–15]	7% [3]	nd	nd	nd	no bands
Chronic obstructive bronchitis (n = 19)	5% [14]	26% [12–14,18]	100% [1–19]	37% [4–6,10,13,15,18]	5% [15]	21% [4–6,9]	no bands	no bands

Percent of subjects with bands and identification numbers of those subjects in whom bands reacting with the anti-pro-SP-B-antibodies CTERMB, NFLANK, CFLANK and NFPROX displaced by the CTERMB, CFLANK, NFLANK or NFPROX peptides, or the anti-pro-SP-C-antibody NPRO-SP-C-C2 and displaced by the respective peptide, were identified. The identification numbers of the patients are given in square brackets []. Numbers in bold refers to bands not identified by the CTERMB antibodies. Due to shortage of lavage material in the normal controls (no lung disease), not all 4 antibodies were tested in this group (nd = not done).

ISSN: 1465-993X