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Figure 3 | Respiratory Research

Figure 3

From: Expression profiles of hydrophobic surfactant proteins in children with diffuse chronic lung disease

Figure 3

SP-B deficiency. Western blotting of a lavage from patient SP-B 06 homozygous for the 121ins2 SFTPB mutation. After SDS-PAGE and transfer, the membranes were probed with the antibodies indicated. The pro-forms were probed in the absence (-) and presence (+) of an excess of the peptide used to raise this antibody. Note that bands that are not displaced by the competing peptide were not considered as specific bands (marked by an asterisk). The numbers next to the lanes indicate the molecular weight in kDa. The closed arrowheads indicate the absence of SP-B and of proforms of SP-B. Arrows show the presence of SP-C (open arrow) and of abberant pro-SP-C (closed arrows). Some aberrant pro-SP-C can also be seen on the SP-C blot, above the SP-C band, which is indicated by an open arrowhead. All bands were analyzed under reducing conditions.

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