Figure 4
From: Cigarette smoke induces IL-8, but inhibits eotaxin and RANTES release from airway smooth muscle
Effect of CSE or TNFα on (A) RANTES and (B) eotaxin release from HASMC. Cells were incubated in the presence and absence of CSE (10 %) or TNFα (1 ng/ml) for 24 hours. Effect of CSE on TNFα-induced (C) RANTES and (D) eotaxin release from HASMC. Cells were stimulated with CSE concentrations from 5–30% for 24 hours in presence of TNFα (1 ng/ml). Cell free supernatant was assessed for RANTES and eotaxin by ELISA. Cells from 3 different donors were used for the experiments. Data are expressed as mean ± SEM.