Figure 2
From: Cigarette smoke induces IL-8, but inhibits eotaxin and RANTES release from airway smooth muscle
(A) Effect of glutathione (GSH) on cigarette smoke-induced IL-8 release from HASMC. Cells were pretreated with 100 μM GSH for 30 min before adding CSE (10%). Data are expressed as mean ± standard error of the mean (SEM). (B) CSE induced heme oxygenase-1 (HO-1) expression in HASMC. Cells were exposed to CSE (0–20%) for 24 hours. HO-1 expression was detected by western blotting. The blot shown in the upper panel was stripped and reprobed using a GAPDH antibody to show equal protein loading. A representative example of three identical experiments is shown. In the lower panel densitometric analysis of HO-1 expression, normalized by GAPDH expression, is shown.