Figure 1
From: Cigarette smoke induces IL-8, but inhibits eotaxin and RANTES release from airway smooth muscle
Effect of increasing concentrations of CSE on IL-8 production from HASMC. (A) Cells were stimulated with CSE concentrations from 5–30% for 24 hours. Cell free supernatants were assessed for IL-8 by ELISA. n = 3 from 1 donor. Similar results were obtained from 2 other donors. *** p < 0.001; * p < 0.05 compared to untreated cells. (B) Effect of CSE (10%) on IL-8 mRNA expression in HASMC. Cells from 4 different donors were used for the experiments. Data were normalized to GAPDH expression and are expressed as mean ± SEM. (C) HASMC viability in the presence of CSE (0–30%) was assessed by using the MTT test. Results are expressed as percentage of untreated control cells (mean ± SEM, n = 3).