Figure 6
From: Predominant constitutive CFTR conductance in small airways
Effect of Cl- conductance agonists on TEP: Applying Fsk (5 μM)+IBMX (100 μM), adenosine (100 μM), ATP (100 μM) and ATP (100 μM)+adenosine (100 μM) to the perfusate to activate CFTR gCl- in the presence of a hyperpolarizing Cl- gradient, the TEP hyperpolarized significantly; however, the response appeared to be increased when both ATP and adenosine were added together. In order to observe maximum effect on activation of Cl- conductance, amiloride (10 μM) is present in all luminal perfusate above to block Na+ conductance.