Figure 1

Pipette assembly for microperfusing segments of undissected bronchiole. The bronchiole is held in outer large pipette (A) by suction. An inner, septated cannulating pipette provides current passing capacity through one barrel (B₁) and perfusing fluid to the duct lumen through the opposite barrel (B₂), which also contains a small cannula pipette (C) that allows changes of perfusing solutions.