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Figure 5 | Respiratory Research

Figure 5

From: Alveolar macrophages regulate neutrophil recruitment in endotoxin-induced lung injury

Figure 5

A. Total cell count in bronchoalveolar lavage fluid (BALF). Rats were challenged with intratracheal instillation of LPS or PBS, followed by intratracheal application of MCP-1 antibody or a control antibody (IgG). Lungs were evaluated 4 hours later. Cells were analyzed using cytospin and Diff-Quick staining. Values are mean ± SEM from 5 animals. B. Determination of myeloperoxidase (MPO) activity as a measure of parenchymal PMN content. Rats were challenged with intratracheal instillation of LPS or PBS, followed by intratracheal application of MCP-1 antibody or a control antibody (IgG). Lungs were evaluated 4 hours later. The right lung of each animal was removed and homogenized in sample buffer. Optical density was measured at 420 nm over 360s. One value PBS+IgG was defined as 1 and all other values were adapted. Values are mean ± SEM from 5 animals. C. Total cell count in bronchoalveolar lavage fluid (BALF). AM-competent (control liposomes = co lip) and -depleted (clodrondate) animals were challenged with intratracheal instillation of LPS, followed by intratracheal application of MCP-1 antibody or a control antibody (IgG). Lungs were evaluated 4 hours later. Cells were analyzed using cytospin and Diff-Quick staining. Values are mean ± SEM from 5 animals. D. Determination of myeloperoxidase (MPO) activity as a measure of parenchymal PMN content. AM-competent (control liposomes = co lip) and -depleted (clodrondate) animals were challenged with intratracheal instillation of LPS, followed by intratracheal application of MCP-1 antibody or a control antibody (IgG). Lungs were evaluated 4 hours later. The right lung of each animal was removed and homogenized in sample buffer. Optical density was measured at 420 nm over 360s. One value LPS+IgG was defined as 1 and all other values were adapted. Values are mean ± SEM from 5 animals.

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