Figure 4
From: Alveolar macrophages regulate neutrophil recruitment in endotoxin-induced lung injury
A. TNF-α protein determination in supernatants of alveolar epithelial cells (AEC), alveolar macrophages (AM) and co-culture of AEC/AM upon LPS stimulation (relation AEC:AM = 2:1). TNF-α was examined using a standard ELISA. White bars represent control cells, black bars LPS-stimulated cells. Values are mean ± SEM from 5 different assays. B. MCP-1 protein determination in supernatants of alveolar epithelial cells (AEC), alveolar macrophages (AM) and co-culture of AEC/AM upon LPS stimulation (relation AEC:AM = 2:1). MCP-1 was examined using a standard ELISA. White bars represent control cells, black bars LPS-stimulated cells. Values are mean ± SEM from 5 different assays. C. MIP-1β protein determination in supernatants of alveolar epithelial cells (AEC), alveolar macrophages (AM) and co-culture of AEC/AM upon LPS stimulation (relation AEC:AM = 2:1). MIP-1β was examined using Western blot analysis. Densitometry was performed. One value for control liposomes and PBS was defined as 1 and all other values were adapted. White bars represent control cells, black bars LPS-stimulated cells. Values are mean ± SEM from 5 different assays.