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Figure 2 | Respiratory Research

Figure 2

From: Alveolar macrophages regulate neutrophil recruitment in endotoxin-induced lung injury

Figure 2

A. TNF-α and protein determination in bronchoalveolar lavage fluid of AM-competent (control liposome = co lip) and AM-depleted (clodronate) animals, exposed to PBS or LPS for 4 hours. Proteins were electorphoresed on a SDS-polyacrylamide gel and transblotted to a nitrocellulose membrane. Densitometry was performed. One value for control liposomes and PBS was defined as 1 and all other values were adapted. Values are mean ± SEM from 5 animals (only 2 are shown). B. MCP-1 protein determination in bronchoalveolar lavage fluid of AM-competent and -depleted animals, exposed to PBS or LPS for 4 hours. Proteins were electorphoresed on a SDS-polyacrylamide gel and transblotted to a nitrocellulose membrane. Densitometry was performed. One value for control liposomes and PBS was defined as 1 and all other values were adapted. Values are mean ± SEM from 5 animals (only 2 are shown). C. MIP-1β protein determination in bronchoalveolar lavage fluid of AM-competent and -depleted animals, exposed to PBS or LPS for 4 hours. Proteins were electorphoresed on a SDS-polyacrylamide gel and transblotted to a nitrocellulose membrane. Densitometry was performed. One value for control liposomes and PBS was defined as 1 and all other values were adapted. Values are mean ± SEM from 5 animals (only 2 are shown). D. MIP-2 protein determination in bronchoalveolar lavage fluid of AM-competent and -depleted animals, exposed to PBS or LPS for 4 hours. Proteins were electorphoresed on a SDS-polyacrylamide gel and transblotted to a nitrocellulose membrane. Densitometry was performed. One value for control liposomes and PBS was defined as 1 and all other values were adapted. Values are mean ± SEM from 5 animals (only 2 are shown). E. CINC concentration in bronchoalveolar lavage fluid (BALF) of AM-competent (control liposomes = co lip) and AM-depleted (clodronate) animals, exposed to PBS or LPS for 4 hours. CINC was examined using a standard ELISA. Values are mean ± SEM from 5 animals.

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