Figure 1

A: Total cell count in bronchoalveolar lavage fluid (BALF). Animals were pretreated with control liposomes (co lip) or clodronate-liposomes (clodronate). 72 hours later 150 μg LPS was instilled intratracheally and lungs were evaluated 4 hours later. Cells were analyzed using cytospin and Diff-Quick staining. Values are mean ± SEM from 5 animals per group. B. Determination of myeloperoxidase (MPO) activity as a measure of parenchymal neutrophil content. Animals were pretreated with control liposomes (co lip) or clodronate-liposomes (clodronate). 72 hours later 150 μg LPS was instilled intratracheally and lungs were evaluated 4 hours later. The right lung of each animal was removed and homogenized in sample buffer. Optical density was measured at 420 nm over 360s. The value for control liposomes and PBS was defined as 1 and all other values were adapted. Values are mean ± SEM from 5 animals per group.