Figure 6

Representative double-labeled confocal fluorescent images of staining for epimorphin plus keratin (A), epimorphin (C), vimentin (D), and epimorphin plus vimentin (E) in an early fibrotic lesion from the lung of a patient with NSIP. (A) The presence of epimorphin (green) in areas of early fibrosis and the presence of keratin (red) in epithelial cells overlying the lesions were confirmed. (B) The same image shown in part A but with Nomarski optics used. (C–E) Epimorphin (C, green) localized in vimentin-positive (D, red) stromal cells (yellow-orange) and in surrounding ECM in early fibrotic areas (E). Nuclei were 4,6-diamidino-2-phenylindole dihydrochloride-positive (blue). Asterisks indicate early fibrotic areas. Scale bars = B: 20 μm, E: 5 μm.