Figure 5

Immunohistochemical localization of nitrotyrosine in the lung. No positive staining was observed in the lung section for sham-treated mice (A). After bleomycin injection in iNOSWT mice, positive staining for nitrotyrosine (B) was localized mainly in nuclei of inflammatory cells. There was a marked reduction in the immunostaining in the lungs of bleomycin-treated iNOSKO mice (C) and in the lungs of bleomycin-treated iNOSWT mice which received GW274150 (D). Original magnification: 150×. This figure is representative of at least 3 experiments performed on different experimental days.