Figure 9
From: TGF-β1 induces human alveolar epithelial to mesenchymal cell transition (EMT)
Effect of Smad2 siRNA on TGF-β1 induced EMT in A549 cells. Pooled synthetic siRNA duplexes targeting different regions of Smad2 were transfected into A549 cells at 50 pmol per well. 24 h after transfection, cells were stimulated with 5 ng/ml of TGF-β1 in serum free 0.1% BSA/DMEM for a further 48 h prior to harvest. Equal amounts of lysates were resolved by SDS-PAGE and analyzed by Western blotting for expression of proteins.