Figure 3

Comparison of EMT-related marker expression in response to TGF-β1, IL-1β and TNF-α treatments. A549 cells were incubated with TGF-β1, IL-1β and TNF-α at the indicated concentrations for 48 h. Only TGF-β1 decreases E-cadherin expression concomitant with increasing Fn-EDA expression. In contrast, TNF-α only slightly decreases E-cadherin expression and IL-1β has no influence on EMT-related marker expression. Equal amounts of total protein are loaded in each lane.