Mevastatin inhibited C. pneumoniae-mediated Rac1, RhoA and TLR4, expression. (A) Representative CLSM images of type II cells pre-treated and non-treated with mevastatin followed by C. pneumoniae incubation for 30 min. Detection with antibody for Rac1, RhoA and TLR4 followed by Alexa594-labeled secondary antibody (red). Labelled for F-actin with Alexa488-conjugated phalloidin (green) and nuclear DNA with DAPI (blue). The red labeled Rac1, RhoA and TLR4 appear yellow when co-localized with green labeled F-actin. The bar equals 10 μm. (B) Rac1, RhoA and TLR4 were visualized by Western blot technique and (C) their expression in membrane fractions was determined by densitometry in control cells (lane 1), C. pneumoniae incubated cells for 30 min in absence (lane 2) and in presence of mevastatin (lane 3). The values are means ± SD in arbitrary units of n = 3 experiments. Open bars, Rac1; solid bars, RhoA; hatched bars, TLR4. Asterisk indicates a significant difference compared to controls (1) and mevastatin (3) (P < 0.05).