Table 2 Effects of SKF96365 and La³⁺ alone and in combination with DHS or DMS on the muscarine-induced reductions in luminal airway area in FVB mice Effects of the Ca²⁺-entry inhibitors La³⁺ (10^-6 M) and SKF 96365 (10^-5 M) alone and in combination with DHS or DMS (both 10^-6 M) on the muscarine induced reduction in luminal airway area (expressed in %). The number of experiments (lungs/slices), mean airway diameter in μm, and the luminal airway area determined 1 min (1) and 15 min (2) after stimulation with muscarine and 1 min (3) and 15 min (4) and after stimulation with muscarine (Mus) in combination with La³⁺ (Mus/La³⁺) or SKF96365 (Mus/SKF), or after stimulation with muscarine and La³⁺ or SKF96365 in combination with DHS or DMS are shown. In all experiments, the muscarine concentration was 10^-6 M. Data are given as means ± S.E.M. Matched pairs (1 vs. 3; 2 vs. 4) were evaluated by Wilcoxon's rank sum test. Differences were considered as statistically significant when p < 0.05.

From: Activation of the SPHK/S1P signalling pathway is coupled to muscarinic receptor-dependent regulation of peripheral airways