Figure 2

Expression of SPHK1 in PCLS from different mouse strains studied via confocal laser scanning microscopy. Representative confocal laser scanning micrographs of double labeling immunohistochemistry demonstrates the restriction of SPHK1-immunoreactivity to smooth muscle cells, identified by immunoreactivity for the marker protein α-smooth muscle actin (α-sma). SPHK1a-immunoreactivity was present in peripheral airway smooth muscle of knock-out animals (M₂R-KO, a-d) and wild-type (M₂R-wt, e-h, FVB, i-l). Granular immunoreactivity could be detected in the cytoplasm and in the membrane of smooth muscle cells. Bars = 50 μm