Figure 4

Histologic findings. (A) H&E stain. Lung sections from mice that received spleen cells from OVA-sensitized mice (TROVA) and from mice that received OVA-sensitization and aerosol OVA-challenge (OVA/OVA) are shown. Scale bar, 200 μm. (B) Fluorescence study. Spleen cells from OVA-sensitized mice were labeled with fluorescent dye (PKH67), and then transferred into recipients. Five-micrometer sections of the lungs were observed by fluorescence microscopy 24 hours after the transfer (TROVA). A lung section from naive mice without transfer is shown (naive). Scale bar, 100 μm. (C) Double staining analysis of a single section by immunohistochemistry. T cells were detected by staining for CD3 (cytoplasm, blue). Proliferation was assessed by staining for PCNA (nucleus, brown). Lung sections from mice that received spleen cells from OVA-sensitized mice (TROVA) and from mice that received spleen cells from SA-treated mice (TRSA) are shown. Proliferating T cells were clearly detected (black arrow). Scale bar, 40 μm. (D) Histologic findings of colon (H&E stain). Colon sections from mice that received spleen cells from OVA-sensitized mice (TROVA) and from SA-treated mice (TRSA) are shown. Scale bar, 200 μm. (E)Transferred cells recruit into the colon. Spleen cells from OVA-sensitized mice were labeled, and transferred. Five-micrometer sections of the colons were observed by fluorescence microscopy 24 hours after the transfer (TROVA). A colon section from naive mice without transfer is shown (naive). Scale bar, 100 μm.