Figure 1

Passive cell transfer of spleen cells from OVA-sensitized mice induces airway hyperresponsiveness (AHR). (A) Transfer of spleen cells from OVA-sensitized mice induces a moderate AHR. Mice were sensitized with OVA or SA on days 0 and 11. On day 18, recipients received the spleen cells (5 × 10⁷) from SA (without alum)-treated mice (TRSA), OVA-sensitized mice (TROVA), or SA (with alum)-treated mice (TRAlum). Airway responsiveness (AR) to methacholine (Mch) was measured with Penh methods on day 22 (4 days after the transfer) as described in Methods. Some OVA-sensitized mice were inhaled with OVA from day 18 to day 20. AR was measured on day 18 in mice received that i.p SA injection (SAip) or OVA injection only (OVAip), or on day 21 in mice that received OVA-sensitization and -inhalation (OVA/OVA). Values are presented as means ± SEM for 5 to 14 mice per group. * p < 0.05 compared with PC₂₀₀Mch of TRSA (5 × 10⁷). # p < 0.05 compared with PC₂₀₀Mch of SAip. (B) AR to Mch increases in a transferred-cell-number-dependent manner (n = 5–14 per group). Recipients received spleen cells from SA-treated mice (TRSA; 5 × 10⁷) or OVA-sensitized mice (TROVA; 1 × 10⁶, 5 × 10⁶, 2 × 10⁷, or 5 × 10⁷). AR was measured 4 days after the transfer. * p < 0.05 compared with PC₂₀₀Mch of TRSA (5 × 10⁷). (C) AR was assessed by measurement of airway resistance (Raw) (n = 6–12 per group). * p < 0.05 compared with PC₂₀₀Mch Raw of TRSA (5 × 10⁷). # p < 0.05 compared with PC₂₀₀Mch Raw of SAip.