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Table 1 IC50 values for the inhibition of cytokine mRNA synthesis by different MAPK inhibitors in CD28 or ICOS costimulated human CD4+ T cells. Human CD4+ T cells were preincubated with increasing concentrations of SP600125, U0126 and SB203580. The cells were then stimulated with beads coated with α-CD3/B7-2 fusion protein or α-CD3/B7-H2 fusion protein for 8 hours. Cytokine mRNA level were determined in triplicates using real time RT-PCR and were normalized to β-actin. Stimulated cells treated with DMSO were used as 100% control. IC50values were calculated for the different cytokines. Results represent mean ± s.e.m. for three different donors.

From: Inhibitors of mitogen-activated protein kinases differentially regulate costimulated T cell cytokine production and mouse airway eosinophilia

  α-CD3/B7-2 stimulation
μM IL-2 IL-4 IL-13 IFNγ
U 0126 1.17 ± 0.97 no effect* 0.18 ± 0.14 0.72 ± 0.39
SB 203580 6.99 ± 4.57 no effect* 3.02 ± 1.14 4.15 ± 1.38
SP 600125 no effect* no effect* no effect* no effect*
  α-CD3/B7-H2 stimulation
μM IL2 IL4 IL13 IFNγ
U 0126 0.11 ± 0.03 1.29 ± 0.96 0.31 ± 0.09 0.14 ± 0.08
SB 203580 no effect* no effect* no effect* 6.93 ± 2.47
SP 600125 1.25 ± 0.18 no effect* 1.33 ± 0.36 0.79 ± 0.35
  1. *concentrations up to 30 μM were tested