Figure 6
Hyperoxia-induced lung injury, lung edema and vascular permeability. Parameters of lung damage were considered under basal conditions and following hyperoxia. (A) Lung injury scores from IGF-1R+/+ (n = 4) and IGF-1Rneo/- (n = 4) mice under basal conditions (normoxia) and under hyperoxia. A cumulative score was based on evaluation of hemorrhage and intraalveolar deposition (6 random sections per lung). (B) Quantitated perivascular edema from IGF-1R+/+ (n = 4) and IGF-1Rneo/- (n = 4) mice under basal conditions (normoxia) and under hyperoxia. Surface of perivascular edema was reported to the surface of the vessel, and noted SE/SV (5 selected sections per lung). (C) Lung edema was determined as mean values (± SD) of wet-to-dry lung weight ratio under basal conditions (normoxia) in IGF-1R+/+ (n = 4) and IGF-1Rneo/- (n = 4) mice, and under hyperoxia in IGF-1R+/+ (n = 7) and IGF-1Rneo/- (n = 8) mice. (D) Evans Blue dye extravasation was assessed as mean values (± SD) in IGF-1R+/+ (n = 13) and IGF-1Rneo/- (n = 11) mice. Crosses indicate significant differences between hyperoxic and normoxic values in IGF-1R+/+ mice, and asterisks indicate significant differences between IGF-1R+/+ and IGF-1Rneo/- mice under hyperoxic conditions; P < 0.05.