Agarose gel showing α7 nicotinic acetylcholine receptor expression in SCLC cell lines. cDNA was amplified by PCR using the human α7 primers. SCLC cell lines: 1) WBA; 2) NCI-H69; 3) NCI-H82; 4) NCI-H146; 5) NCI-H187; 6) NCI-H209; 7) NCI-H526. For all gene expression experiments, negative control reactions were performed and found to be negative. The bands were consistent with the expected size, 335 bp. M-100 bp DNA ladder.