Figure 8
From: TGF-β1 and serum both stimulate contraction but differentially affect apoptosis in 3D collagen gels
TUNEL positivity and Western blot of selected pro-apoptotic and anti-apoptotic factors in murine fibroblasts and HFL-1 cells with or without TGF-β1. After staining, TUNEL positive cells as a % of total cells were counted under the microscope in 5 high-power fields. Panel A: Murine Smad3 KO and control cells; Panel B: HFL-1 cells ± siRNAs. Vertical axis: TUNEL positivity expressed as % of positive control (DNAse treated). Horizontal axis: condition. TGF-β1 increased TUNEL positivity in all cell types except in S3KO cells (Panel A) and Smad3 siRNA cells (Panel B). *p < 0.05, as compared with control. Data are shown as means ± SEM. Data presented are from one representative experiment of three experiments performed on separate occasions.