Figure 7

CSC-induced phosphorylation of p38 and JNK. NCI-H292 cells were stimulated with medium, TGF-α, TNF-α or CSC (10, 5 and 1 AU/ml) for 10 minutes, 1 and 6 hours. Next cellular lysates were prepared and used to detect the level of total (t-p38 and t-JNK) and phosphorylated (p-38 and t-JNK) p38 and JNK using Western blot analysis. The results shown in each panel are from one experiment that was repeated three times with similar results.