Skip to main content
Figure 3 | Respiratory Research

Figure 3

From: Effects of cigarette smoke condensate on proliferation and wound closure of bronchial epithelial cells in vitro: role of glutathione

Figure 3

Effect of N-acetylcysteine (NAC) on CSC-induced epithelial wound repair in NCI-H292 and in PBEC. Mechanical wounds prepared in monolayers of NCI-H292 (panel A) or PBEC (panel B). Following wounding, the cultures were incubated for 24 h (NCI-H292; panel A) or 8 h (PBEC; panel B) with freshly prepared CSC (5 or 1 AU/ml), TGF-α (20 ng/ml), medium alone, NAC (1 mM) (alone or in combination with CSC). Next the residual wound area was determined and used to calculate the % wound repair. Similar results were obtained when analyzing wound closure at 72 h (data not shown). The results in NCI-H292 cells are mean ± SEM of 3 independent experiments, each performed in triplicate. The results in panel B are mean ± SEM of PBEC cultures derived from 5 different donors. The cultures from the different donors were performed on different days, and each experiment was performed in triplicate. * p < 0.05 vs. medium alone; + p < 0.05 vs. CSC 5 AU/ml

Back to article page