Figure 3
From: Marked alveolar apoptosis/proliferation imbalance in end-stage emphysema
Gel-electrophoresis: a) Oligonucleosomal-length DNA laddering in emphysematous and control lungs. Lane 1: DNA marker; Lanes 2–5: control donor lungs (4 cases); Lanes 6–9: AAT-deficiency emphysema patients (4 cases); Lanes 10–13: smoking-related emphysema patients (4 cases); Lane 14: positive control. b) Quantification of DNA laddering based on scanning densitometry of bands approximately between 1000 bp and 300 bp (arrowhead) followed by normalization with the density obtained with the equivalent band of the thymus DNA positive control (lung sample/control = densitometric ratio) which was included in every oligonucleosomal DNA laddering assay.