Invasive versus noninvasive measurement of allergic and cholinergic airway responsiveness in mice

Respiratory Research

Table 1 Baseline values for respiratory parameters from allergic and control BALB/c mice

Respiratory parameters	Definition	Control mice conscious	Allergic mice conscious	Control mice anesthetized	Allergic mice anesthetized
VT, ml	tidal volume	0.21 ± 0.05	0.19 ± 0.04	0.14 ± 0.02	0.13 ± 0.02
f, breaths/min	respiratory frequency	198 ± 41	220 ± 23	129 ± 20*	124 ± 29*
TE, s	time of expiration	0.17 ± 0.06	0.14 ± 0.02	0.3 ± 0.04*	0.3 ± 0.05*
EF₅₀, ml/s	tidal midexpiratory flow	2.05 ± 0.89	2.26 ± 0.46	0.93 ± 0.14*	1.12 ± 0.43*
GL, ml·s^-1·cmH₂O^-1	pulmonary conductance	-	-	1.05 ± 0.36	1.29 ± 0.69
Cdyn, ml·cmH₂O^-1	dynamic compliance	-	-	0.037 ± .007	0.030 ± .008

Baseline values are means ± SD obtained from 8 animals per group during a 5 min control period from conscious and anesthetized, orotracheally intubated BALB/c mice. In comparison with conscious mice, EF₅₀, TE and f values were significantly altered in anesthetized mice. No difference was found between allergic animals and control groups when separated into conscious and anesthetized mice. *P < 0.05 versus conscious mice.

ISSN: 1465-993X