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Figure 1 | Respiratory Research

Figure 1

From: Characterization of lymphocyte populations in nonspecific interstitial pneumonia*

Figure 1

Immunohistochemical localization of CD3, CD4, CD8, CD20 and CD68 in lung tissue with NSIP. Bound primary antibodies to these antigens were detected by an avidin-biotin immunoperoxidase method, with AEC substrate (arrow) and counterstained with 1% hematoxylin. Staining for CD3 was present on lymphocytes located throughout the interstitium and lymphoid follicles. CD4 cells were primarily present in a perifollicular location. CD8 expressing cells were scattered throughout the interstitium. CD20 was also primarily localized to the follicles. The CD68 macrophage/monocyte marker was found in clusters of the intra-alveolar spaces and on occasional individual cells within the interstitium. A. Percentage of cells stained with each individual antibody, expressed as a percentage of mononuclear cells within the interstitium. Values are reported as mean ± SEM (N = 10 patients).

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