Figure 4

Mem-TNF mice succumb of uncontrolled chronicMtbinfection after 4–6 months. Survival (A) of infected mice (Mtb 100 CFU given by intranasal route), data two independent experiments (n = 16 per group). Organs weights (B) and bacterial loads (CFU) in lungs (C) and spleen (D) were determined at 112 days after infection. The results are expressed as the mean ± SD (n = 6 per group). Microscopic changes of the lung from mem-TNF and WT mice at 112 days (E). Microscopic analysis of the lung reveal a more abundant macrophages and lymphocyte infiltration in mem-TNF mice with confluent foci and less defined granulomatous lesions. Representative haematoxylin and eosin stained lung sections at low (50×) and high power (200×) are shown (n = 6 per group).