Figure 3

Augmented CD4 and CD8 T cell recruitment, pulmonary IFNγ production and antigen-specific T cell response in infected TNF-KO mice are corrected in mem-TNF mice. Recruitment of CD4 (A) and CD8 T cells (B) in the lung of WT, mem-TNF and TNF-KO mice infected with Mtb (100 CFU). Lymphocytes were obtained form lungs from Mtb infected mice at 30 days post infection as described in Materials and Methods. IFNγ levels in lung homogenates at 30 days of infection (C): Increased IFNγ levels in TNF KO mice, which were lower and comparable in mem-TNF and WT mice. Antigen-specific IFNγ production by SupBCG restimulated splenocytes from mem-TNF and TNF-KO mice, but augmented response in TNF-KO splenocytes (D). Data are representative of two independent experiment (n = 4 mice, mean ± SD).