| | MIC (μg/ml) |
|---|
| | hBD-2 | hBD-3 | hBD-4 |
|---|
Organism | H-salt | L-salt | H-salt | L-salt | H-salt | L-salt |
|---|
E. coli
| 26.6 ± 7.6 | 1.1 ± 0.7 | 5.7 ± 2.6 | 4.1 ± 0.8 | 147 ± 31 | 9.1 ± 3.5 |
P. aeruginosa
| 11.6 ± 1.6 | 1.0 ± 0.5 | 0.6 ± 0.2 | 0.6 ± 0.2 | >500 | 1.3 ± 0.6 |
- The bacteria were incubated with defensin in concentrations ranging tenfold in steps from 0.1 to 1000 μg/ml. To measure antibacterial activity more precisely, some values were determined by repeating the analysis with defensin concentrations that ranged in twofold steps from 0.625 to 40 μg/ml. Two incubation media conditions were tested: H-salt was a high salt condition (Na+ 137 mEq/L, Cl- 130 mEq/L, K+ 4.2 mEq/L, osmolarity 270 mOsm/kg, pH 7.4), and L-salt was a low salt condition (Na+ 95 mEq/L, Cl- 90 mEq/L, K+ 25 mEq/L, osmolarity 210 mOsm/Kg, pH 7.1). Values represent the means ± SD of three experiments. (MIC: minimum inhibitory concentration)
