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Figure 5 | Respiratory Research

Figure 5

From: Influence of hypoxia on the domiciliation of Mesenchymal Stem Cells after infusion into rats: possibilities of targeting pulmonary artery remodeling via cells therapies?

Figure 5

In-vivo engraftment and viability controls. GFP signals were researched by confocal microscopy on lungs frozen sections. In a first step, GFP-labeled MSCs were directly injected in ex-vivo excised lungs in order to provide positive control (A, arrow) for confocal images interpretation whereas a non-injected freshly harvested lung served as negative control (B). Then, MSCs were directly injected in the right lower lobe of the lung in vivo and rats placed in normoxic or hypoxic conditions for three weeks. Frozen sections of lungs were observed after three weeks in confocal microscopy to provide in vivo positive engraftment and viability controls. Indeed, the injection site was visualized macroscopically (C, arrows) and GFP signals were seen centered on the injection injury (D, arrows). Bar = 50 μm, a indicates artery.

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