Figure 5
The effects of P2 receptor antagonists on oscillating current amplitude and frequency. The effects of suramin and PPADS (10-4 M) on (a) the amplitude and (b) the frequency of oscillating inward currents induced by ATP, UTP and UDP (10-4 M) in SPA dissociated smooth muscle cells, voltage-clamped at -50 mV, are shown. The agonist was applied for 2 min and then suramin or PPADS was coapplied for a further 2–3 min. The current amplitude was measured for 1 min immediately before and after antagonist addition and average values calculated. The % decrease in amplitude was then calculated as the difference in the 2 average values. The control data were obtained over the same time-course in cells where agonist alone was added. The average frequency of oscillations was also measured for 1 min immediately before and after antagonist addition and compared directly. Vertical lines show s.e.mean. The number of cells is shown in parentheses.