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Figure 1 | Respiratory Research

Figure 1

From: Alcohol reversibly disrupts TNF-α/TACE interactions in the cell membrane

Figure 1

Panel A: Acute EtOH reversibly suppresses TNF-α secretion in transfected A549 cells. A549 cells transfected with the hTNF-α cDNA were re-seeded 24 hours after transfection and subjected to the addition of 0, 50 or 100 mM EtOH for 1 hour (n = 6, *. Denoted p < 0.05). For the washout experiments, a subgroup of transfected cells had media changed after one hour to fresh media without alcohol for an additional hour (n = 6, * denotes p < 0.05). Panel B: Effect of one to four carbon alcohols on TNF-α secretion (n = 4–6, * denotes p < 0.05 compared to EtOH). Panel C: Effect of one to four carbon alcohols on cell-associated TNF-α levels (n = 4–6, * denotes p < 0.05 compared to EtOH). Transfected A549 cells were treated as in Materials and Methods and TNF-α was measured in cell lysates as the cell-associated level (all data are per mg of protein). Panel D: Effect of one to four carbon alcohols on TNF processing as assessed by the ratio of shed vs. cell-associated TNF-α (n = 4–6, * denotes p < 0.05 compared to EtOH). Transfected A549 cells were treated as in Materials and Methods and TNF-α was measured in cell lysates and media and graphed as the ratio of the shed over the cell-associated level.

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